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L1210

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編號:B164934

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產品名稱	L1210
商品貨號	B164934
Organism	Mus musculus, mouse
Tissue	skin
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphocytic leukemia
Age	8 months
Gender	female
Strain	DBA subline 212
Storage Conditions	liquid nitrogen vapor phase
Karyotype	modal number = 40; range = 32 to 80. Mouse karyotype with a diploid stemline number of 40 chromosomes.
Derivation	The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether.
Clinical Data	female
Tumorigenic	Yes
Effects	Yes, in nude mice
Comments	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 ⁴ viable cells/mL. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C
Population Doubling Time	8 to 10 hrs
Name of Depositor	EM Jensen
Deposited As	Mus musculus
References	Law LW, et al. Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. J. Natl. Cancer Inst. 10: 179-192, 1949. PubMed: 18143260 Moore GE, et al. Suspension Cell Culture and In Vivo and In Vitro Chromosome Constitution of Mouse Leukemia L1210. J. Natl. Cancer Inst. 36: 405-421, 1966. PubMed: 18630317 Himmelfarb P, et al. Growth of Colonies of. Murine Leukemia L1210 in Vitro. Cancer Chemother. Rep. 51: 451-3 1967 Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865

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