| 產品名稱 |
L5178-R (LY-R) |
| 商品貨號 |
B164946 |
| Organism |
Mus musculus, mouse |
| Tissue |
thymus |
| Cell Type |
chemically induced |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
DBA/2 |
| Applications |
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tested and found negative for ectromelia virus. |
| Derivation |
This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse. The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line. |
| Tumorigenic |
Yes |
| Effects |
Yes, in DBA/2 mice |
| Comments |
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD). After 6 months to 3 years in culture, the line converts to an X-ray sensitive state. Tested and found negative for ectromelia virus. |
| Complete Growth Medium |
Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10 4 cells/mL and maintain between 3 x 10 4 and 7 x 10 4 cells/mL.
Medium Renewal: Twice per week |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Name of Depositor |
JZ Beer |
| Deposited As |
Mus musculus |
| References |
Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899
Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332
Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312
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