| 產(chǎn)品名稱(chēng) |
LA 3-5 |
| 商品貨號(hào) |
B164951 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Applications |
The cells are resistant to methotrexate. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data |
female |
| Comments |
The cells are resistant to methotrexate. |
| Complete Growth Medium |
Alpha minimum essential medium with 0.001 mM methotrexate, 90%; dialyzed heat-inactivated fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Add fresh medium at the time of subculture Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor |
Genetics Institute, Inc. |
| Deposited As |
Cricetulus griseus |
| U.S. Patent Number |
|
