| 產(chǎn)品名稱 |
LMH/2A |
| 商品貨號 |
B164993 |
| Organism |
Gallus gallus, chicken |
| Tissue |
liver; chemically induced; transfected with chicken estrogen receptor gene |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatocellular carcinoma |
| Strain |
Leghorn |
| Karyotype |
Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines: triploid; modal number = 116; six marker chromosomes |
| Derivation |
LMH/2A is a stably transfected cell line constructed in 1989 by R. Binder from the LMH hepatocellular carcinoma cell line (see ATCC CRL-2117) by cotransfection with the plasmid pRSVCEO (containing the chicken estrogen receptor gene driven by the Rous sarcoma promoter) and the pRSVNEO plasmid (which confers G418 resistance).
|
| Receptor Expression |
estrogen receptor, positive |
| Tumorigenic |
Yes |
| Effects |
Yes, forms tumors in nude mice |
| Comments |
LMH/2A cells express high levels of chicken estrogen receptor.
Apolipoprotein (apoII) gene expression is increased by estrogen 300-fold compared with levels in the low receptor expression parent LMH line.
This cell line is estrogen responsive for the expression of endogenous yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.
These genes include apolipoprotein B and apoII as well as vitellogenin.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes, and remove the liquid.
Vessels prepared in this fashion may be used immediately. |
| Complete Growth Medium |
Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
R Binder, DL Williams |
| Deposited As |
Gallus gallus |
| References |
Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000
Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775
Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707
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