| 產品名稱 |
M18/2.a.12.7 (new clone of M18/2.a.8) |
| 商品貨號 |
B165044 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing. The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18) |
| Comments |
Animals were immunized with mouse cytotoxic T cell glycoproteins. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The Mac-1 antigen is a macrophage differentiation antigen associated with type three complement receptor (CR3). The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing. The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta. The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen. Tested and found negative for ectromelia virus (mousepox). The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
rat IgG2a kappa |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226
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