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MC/9

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編號:B165096

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產品名稱	MC/9
商品貨號	B165096
Organism	Mus musculus, mouse
Tissue	liver
Cell Type	mast cell
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	13 days
Strain	(B6 X A/J)F1
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	This is a cloned mast cell line derived from the fetal liver of a (B6 X A/J)F1 mouse.
Receptor Expression	FcERI (Fc of IgE)
Genes Expressed	histamine; leukotrienes
Cellular Products	histamine; leukotrienes
Comments	The cells can be sensitized to specific antigens by incubating them with IgE having the desired antigenic specificity. The cells so coated will release soluble mediators (such as histamine) in response to exposure to the antigen (allergen).
Complete Growth Medium	Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10% Rat T-STIM (Becton Dickenson Catalog No. 354115) and 10% fetal bovine serum.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 10⁵ viable cells/ml. Maintain cell density between 2 X 10⁵ and 2 X 10⁶ viable cells/mL. Do not allow the cell density to exceed 2 X 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Culture Conditions	Temperature: 37°C
Name of Depositor	Dana Farber Cancer Inst.
Deposited As	Mus musculus
U.S. Patent Number	4,559,310
References	Cantor HI, Nabel G. Assay methods and systems utilizing mast cell clones. US Patent 4,559,310 dated Dec 17 1985 Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154 Galli SJ, et al. Mast cell clones: a model for the analysis of cellular maturation. J. Cell Biol. 95: 435-444, 1982. PubMed: 6216259 Lewin I, et al. Aggregation of the FcepisilonRI in mast cells induces the synthesis of fos-interacting protein and increases its DNA binding activity: the dependence on protein kinase c-beta. J. Biol. Chem. 271: 1514-1519, 1996. PubMed: 8576146 Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

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