| 產品名稱 |
MC-SV-HUC T-2 |
| 商品貨號 |
B165115 |
| Organism |
Homo sapiens, human |
| Tissue |
ureter, immortalized epithelial SV40-transformed |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
11 years |
| Gender |
male |
| Applications |
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data |
male |
| Genes Expressed |
uroepithelial keratins |
| Cellular Products |
uroepithelial keratins |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA). While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid. The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative. Stress (such as chemical exposure) could possibly activate the virus. |
| Complete Growth Medium |
Ham's F12 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
Wisconsin Alumni Res. Fndn. |
| U.S. Patent Number |
|
| References |
Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990
|
