| 產(chǎn)品名稱 | MDA-MB-157 |
|---|---|
| 商品貨號 | B165121 |
| Organism | Homo sapiens, human |
| Tissue | mammary gland; breast/medulla |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | medulallary carcinoma |
| Age | 44 years adult |
| Gender | female |
| Ethnicity | Black |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1;12)(q21;p13), der(7)t(7;?)(q22;?), del(1)(q11), t(3qter>3q21::10p15>10qter), 7q+, del(5)(q13), 19q+, t(10q;14q), der(4)t(1;4)(q21;q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Marker chromosome M1 is similar to that described by Q.V. Cruciger, et al., Cytogenet. Cell Genet. 17: 231, 1976. |
| Derivation | The method for derivation of this line was similar in detail to that for ATCC HTB-23. |
| Clinical Data | 44 years adult Black female |
| Antigen Expression | Antigen expression: Blood Type B; Rh- |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice and in immunosuppressed BALB/c mice |
| Comments | The cells express the WNT7B oncogene. Ref
  Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Desmosomes, microvilli and tonofilaments were observed at boundaries between cells. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing | Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 100% Temperature: 37°C |
| STR Profile | Amelogenin: X D13S317: 11 CSF1PO: 10 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1: 7 ,8 TPOX: 11, 9 vWA : 15 |
| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor | R Cailleau |
| Deposited As | Homo sapiens |
| References | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Young RK, et al. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro 9: 239-245, 1974. PubMed: 4471183 Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
