| 產(chǎn)品名稱 | MDA-MB-453 |
|---|---|
| 商品貨號 | B165128 |
| Organism | Homo sapiens, human |
| Tissue | mammary gland/breast; derived from metastatic site: pericardial effusion |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | metastatic carcinoma |
| Age | 48 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 90; range = 87 to 91. The cell line is aneuploid human female, with chromosome counts in the hypo- to near-tetraploid range. Normal chromosomes N11, N13, and N17 are absent, and chromosomes N2, N6, and N12 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N20 tends towards over-representation. A large number of marker chromosomes are present with consistency, many of them with more than one copy. The near-diploid population of cells reported for this culture has been replaced by a tetraploid population. |
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| Derivation | MDA-MB-453 was derived in 1976 by R. Cailleau et al. from an effusion of a 48 year old female patient with metastatic carcinoma of the breast, involving the nodes, brain and both pleural and pericardial cavities. |
| Clinical Data | 48 years
Caucasian
female
|
| Receptor Expression | fibroblast growth factor (FGF), expressed |
| Tumorigenic | No |
| Effects | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments | The cells overexpress FGF receptors. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 100% Temperature: 37.0°C |
| STR Profile | Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 11 D7S820: 10 THO1: 6 TPOX: 10 vWA: 17,18 |
| Isoenzymes | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor | R Cailleau |
| Deposited As | Homo sapiens |
| Year of Origin | June 10, 1976 |
| References | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 McLeskey SW, et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994. PubMed: 7506125 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443 Noonberg SB, et al. Evidence of post-transcriptional regulation of U6 small nuclear RNA. J. Biol. Chem. 271: 10477-10481, 1996. PubMed: 8631843 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
