| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| References |
Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095
. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856
The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome.
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