| 產(chǎn)品名稱 |
MOP-8 |
| 商品貨號(hào) |
B165185 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Cell Type |
SV40 transformed |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
embryo |
| Strain |
NIH/Swiss |
| Applications |
transfection host Transfection host, especially for plasmids containing the polyomavirus origin of replication. |
| Derivation |
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. |
| Comments |
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells will support the replication of recombinant plasmids which contain the polyomavirus origin for DNA synthesis. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor |
JA Hassell |
| Deposited As |
Mus musculus |
| References |
Muller WJ, et al. Isolation of large T antigen-producing mouse cell lines capable of supporting replication of polyomavirus-plasmid recombinants. Mol. Cell. Biol. 4: 2406-2412, 1984. PubMed: 6096696
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