| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| References |
Laskov R, et al. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells. J. Mol. Biol. 56: 327-339, 1971. PubMed: 5102530
Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836
Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146
Blankenstein T, et al. DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171: 965-970, 1990. PubMed: 2106569
Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284
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