| 產品名稱 |
NCI-H1238 [H1238] |
| 商品貨號 |
B165254 |
| Organism |
Homo sapiens, human |
| Product Format |
frozen |
| Culture Properties |
suspension; some adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E, carcinoma; small cell lung cancer (SCLC) |
| Age |
64 years |
| Gender |
male |
| Ethnicity |
White |
| Derivation |
The line was established in November 1985. |
| Clinical Data |
The patient was a smoker. male White 64 years |
| Comments |
The line was established in November 1985. The patient was a smoker. 130 pack years. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters. Some cells will attach. |
| Cryopreservation |
Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11 D13S317: 8,14 D16S539: 10 D5S818: 13 D7S820: 11 THO1: 9,9.3 TPOX: 8 vWA: 18,19 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1985 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
