| 產品名稱 |
NCI-H2087 [H2087] |
| 商品貨號 |
B165311 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: lymph node |
| Product Format |
frozen |
| Morphology |
epithelial-like and/or rounded |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 1, adenocarcinoma; non-small cell lung cancer |
| Age |
69 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in November 1988 from a lymph node metastasis of a lung adenocarcinoma. |
| Clinical Data |
69 years
Caucasian
male
The patient was a smoker
Sixty packs year
|
| Comments |
A lymphoblastoid line from the same patient is available as ATCC® CRL-5965 (NCI-BL2087). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of RPMI 1640 medium supplemented with 5% fetal bovine serum, aspirate cells by gently pipetting and transfer to a centrifuge tube. Spin at 125 x g for 5 to 10 minutes. Discard supernatant.
- Resuspend the pellet in RPMI 1640 medium and dispense into new flasks.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Add fresh medium twice weekly |
| Cryopreservation |
Freeze medium: RPMI 1640 medium, 85%; fetal bovine serum, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D5S818: 11 D13S317: 12 D7S820: 8, 10 D16S539: 9, 11 THO1: 7, 9 TPOX: 11 vWA: 17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
November, 1988 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
