| 產品名稱 |
NRbM |
| 商品貨號 |
B165409 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with rabbit IgM but not with other rabbit immunoglobulin classes. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rabbit IgM |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rabbit IgM |
| Comments |
Animals were immunized with rabbit IgM. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with rabbit IgM but not with other rabbit immunoglobulin classes. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
R Wistar, C Cole |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Cole C, Wistar R Jr.. Monoclonal antibody anti-rabbit IgM, NR6M. Monoclonal Antibody News 7: 15-16, 1989.
|
