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NTERA-2 cl.D1 [NT2/D1]

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編號:B165417

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產品名稱	NTERA-2 cl.D1 [NT2/D1]
商品貨號	B165417
Organism	Homo sapiens, human
Tissue	testis; derived from metastatic site: lung
Product Format	frozen
Morphology	epithelial-like,differentiation changes phenotype
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	malignant pluripotent embryonal carcinoma
Age	22 years
Gender	male
Ethnicity	Caucasian
Applications	This cell line is a suitable transfection host.
Karyotype	This is a hypotriploid human cell line with the modal chromosome number of 63 in 48% of cells examined. However, cells with 62 chromosome counts also occurred at a rather high frequency (24%). The rate of polyploidy was 1.6%., About 12 marker chromosomes are constantly found in most cells. They include: der(9)t(1;9)(q25;q34.3); del(1)(q25); der(13)t(11;13)(q13;q34); t(Xq1q); and eight others., At least two markers are found only in some cells. The normal Y chromosome was found in all cells. Only single copies of normal chromosomes 1, 10, 11 and 13 were present. Others were mostly in two or three copies per cell.
Derivation	The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106). The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line.
Clinical Data	22 years Caucasian male
Virus Resistance	UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
Comments	This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity. To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid. Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Subcultures are prepared by scraping. Cells from confluent cultures (approximately 20 million cells per 75 cm²) are dislodged from the flask surface, aspirated and dispensed into new flasks. Cultures should be maintained at high density. Seed new flasks at a density of at least 5 X 10⁶ viable cells per 75 cm² flask. Medium Renewal: Every 2 to 3 days
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12,13 D5S818: 9,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 18,19
Name of Depositor	PW Andrews
Deposited As	Homo sapiens
Year of Origin	1980
References	Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662 Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410 Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853 Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356 Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286 Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309 Andrews PW. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603 Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047 Dewji NN, Singer SJ. Cell surface expression of the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228 Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenous CRIPTO expression and inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871

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