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The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Yagil C, et al. Insulin binding, internalization, and degradation by a cultured kidney cell line. Am. J. Physiol. 254: E601-E608, 1988. PubMed: 2834959

Cole JA, et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3', 5'-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-2989, 1988. PubMed: 2836179

Nakai M, et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem. Biophys. Res. Commun. 152: 1416-1420, 1988. PubMed: 2837187

Cheng L, et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem. Biophys. Res. Commun. 155: 74-82, 1988. PubMed: 2843189

Koyama H, et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-246, 1978. PubMed: 566717

. . J. Pharmacol. 244: 571-578, 1988.

Nucleotide (GenBank) : X95475 D.virginiana mRNA for rBAT protein.