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P3.6.2.8.1

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編號:B165452

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產品名稱	P3.6.2.8.1
商品貨號	B165452
Organism	Mus musculus, mouse
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Strain	BALB/c
Applications	This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). Tested and found negative for ectromelia virus (mousepox).
Karyotype	near tetraploid
Derivation	This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Antigen Expression	H-2d; PC.1
Genes Expressed	immunoglobulin; monoclonal antibody,H-2d; PC.1
Cellular Products	immunoglobulin; monoclonal antibody
Comments	This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). The cells bear surface immunoglobulin (sIg+). Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
Isotype	IgG1; kappa light chain
Name of Depositor	P Knopf, A Destree, R Hyman
Deposited As	Mus musculus
References	Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

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