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P388D1 (IL-1)

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編號:B165454

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產品名稱	P388D1 (IL-1)
商品貨號	B165454
Organism	Mus musculus, mouse
Cell Type	methylcholanthrene induced
Product Format	frozen
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoid tumor
Strain	DBA/2
Applications	The cells are negative for surface immunoglobulin (sIg-). Tested and found negative for ectromelia virus (mousepox).
Receptor Expression	complement (C3)
Genes Expressed	interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme
Cellular Products	interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme
Comments	These cells phagocytose zymosan and latex beads. They are active in antibody dependent cell mediated cytotoxicity systems. The cells are negative for surface immunoglobulin (sIg-). Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 3 times per week Subcultures are prepared by scraping. Remove old medium, add fresh dislodge the cells and dispense into new flasks.
Culture Conditions	Temperature: 37.0°C
Name of Depositor	S Mizel
Deposited As	Mus musculus
References	Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860 Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564 . Manual of macrophage methodology. New York: Marcel Dekker; 1981.

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