| 產(chǎn)品名稱(chēng) |
QM7 (Quail muscle clone 7) |
| 商品貨號(hào) |
B165544 |
| Organism |
Coturnix coturnix japonica, quail, Japanese |
| Tissue |
muscle |
| Cell Type |
myoblast myoblast; chemically induce |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
fibrosarcoma |
| Age |
1 to 3 weeks |
| Applications |
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin. QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression. To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene. |
| Genes Expressed |
desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form) |
| Cellular Products |
desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form) |
| Comments |
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene. QM7 is a serum inducible myogenic cell line. The cells replicate as myoblasts in medium containing serum. When switched to medium without serum, the cells cease dividing and fuse to form large multinucleated myotubes. In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin. Mucle creatine kinase, myosin light chain 2 and a ventricular form of myosin heavy chain. They do not express any known form of alpha actin. QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression. The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent. To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells. |
| Complete Growth Medium |
Medium 199 with Earle's BSS, 80%; tryptose phosphate broth, 10%; fetal bovine serum, 10%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: 1:4 to 1:5
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor |
PB Antin |
| Deposited As |
Coturnix coturnix japonica |
| References |
Antin PB, Ordahl CP. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143: 111-121, 1991. PubMed: 1985013
Rong S, Sheppard MG. Processes for preparation of Marek's disease virus using continuous avian cell lines. US Patent 6,410,297 dated Jun 25 2002
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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