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Raji

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編號:B165571

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產品名稱	Raji
商品貨號	B165571
Organism	Homo sapiens, human
Tissue	lymphoblast
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Herpesvirus (EBV)] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Burkitt's lymphoma
Age	11 years
Gender	male
Ethnicity	Black
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase
Karyotype	Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra "E" group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome.
Derivation	The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt's lymphoma of the left maxilla of an 11-year-old Black male.
Clinical Data	11 years Black male The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt's lymphoma of the left maxilla of an 11-year-old Black male.
Comments	These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4 x 10⁵ viable cells/mL. A maximum of 3 X 10⁶ viable cells/mL is obtainable. Corning^® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 8,11 D5S818: 10,13 D7S820: 10 THO1: 6,7 TPOX: 8,13 vWA: 16,19
Name of Depositor	W Henle
Deposited As	Homo sapiens
Year of Origin	1963
References	Pulvertaft JV. Cytology of Burkitt's tumour (African lymphoma). Lancet 1: 238-240, 1964. PubMed: 14086209 Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568 Epstein MA, et al. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547-559, 1966. PubMed: 4288580 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Rich SA, et al. Purification, microresequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions. J. Biol. Chem. 271: 1118-1126, 1996. PubMed: 8557639 U.S. Pharmacopeia USP Monographs: Technetium 99mTc Fanolesomab Injection. Rockville, MD: USP32-NF27, 2005

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