| 產(chǎn)品名稱 |
RD-ES |
| 商品貨號(hào) |
B165587 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
mixed: adherent and clusters in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Ewing's sarcoma |
| Age |
19 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Derivation |
The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus. |
| Clinical Data |
19 years Caucasian male |
| Antigen Expression |
Blood type B; Rh+ |
| Comments |
Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter. The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
G Marshall, M Kirchen |
| Deposited As |
Homo sapiens |
