| 產品名稱 |
RLSD09 [Anti-PA 3, DB90 5CT3.5.2] |
| 商品貨號 |
B165611 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified PA from human semen. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human prostate antigen (PA, 33000 dalton glycoprotein) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human prostate antigen (PA, 33000 dalton glycoprotein) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with purified PA from human semen. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM
L-glutamine, 0.56 mM sodium pyruvate, 1.1 mM oxalacetic acid, 20 U/mL bovine
insulin, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
fetal bovine serum, 90%; DMSO, 10% |
| Isotype |
IgG1 |
| Name of Depositor |
Cetus Corp. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
M'Timkulu T, et al. Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay. US Patent 4,690,890 dated Sep 1 1987
|
