| 產(chǎn)品名稱 |
RPMI 8226 |
| 商品貨號(hào) |
B165630 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
plasmacytoma; myeloma |
| Age |
61 years |
| Gender |
male |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres. |
| Clinical Data |
male |
| Antigen Expression |
HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e + |
| Genes Expressed |
immunoglobulin light chain |
| Cellular Products |
immunoglobulin light chain |
| Virus Susceptibility |
Human poliovirus 1
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
|
| Comments |
There is no evidence of heavy chain production (cytoplasmic or secreted). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Do not allow the cell density to exceed 2 to 3 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cell density between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 11 D16S539: 9 D5S818: 11,13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes |
G6PD, A |
| Isotype |
lambda light chain |
| Year of Origin |
June, 1966 |
| References |
Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436
Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066
Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
|
| Cross References |
Nucleotide (GenBank) :
U07992
Human myeloma immunoglobulin lambda light chain mRNA, partial cds.
|
