Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
sarcoma
Strain
Amsterdam
Tumorigenic
Yes
Effects
Yes, in chickens
(Injection of these cells into 2 week old chickens produced rapidly growing tumors, the cells of which possessed chicken chromosomes. The tumors apparently result from low levels of residual Rous sarcoma virus.)
Yes, in newborn rats
(Injection of as few as 10 cells results in rapidly growing tumors.)
Virus Resistance
poliovirus 1, 2, 3
Complete Growth Medium
Medium 199 in Hanks' BSS, 80%; bovine calf serum, 20%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove medium and allow the culture to sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor
WW Nichols
Deposited As
Rattus sp.
References
. . Hereditas 50: 53-80, 1963.
Injection of these cells into 2 week old chickens produced rapidly growing tumors, the cells of which possessed chicken chromosomes. The tumors apparently result from low levels of residual Rous sarcoma virus.
Injection of as few as 10 cells results in rapidly growing tumors.
