| 產品名稱 |
RS4;11 |
| 商品貨號 |
B165633 |
| Organism |
Homo sapiens, human |
| Tissue |
bone marrow |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute lymphoblastic leukemia |
| Age |
32 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The cells have a characteristic chromosomal abnormality, t(4;11)(q21;q23), and an isochromosome for the long arm of chromosome 7. |
| Derivation |
This line was established from the bone marrow of a patient with acute lymphoblastic leukemia. |
| Clinical Data |
32 years
Caucasian
female
|
| Antigen Expression |
HLA DR +; CD9 +; CD24 + |
| Comments |
The cells lack surface and cytoplasmic immunoglobulin, and are negative for CALLA (CD10).
The cells are also negative for myeloperoxidase and chloroacetate esterase and do not stain with Sudan Black. The cells are strongly positive for terminal deoxynucleotidyl transferase (TdT) and are positive for BA-1 (CD24), BA-2 (CD9) and MHC Class II antigens (HLA DR).
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
| Culture Conditions |
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8 vWA: 14,17 |
| Name of Depositor |
JH Kersey |
| Deposited As |
Homo sapiens |
| References |
Stong RC, et al. Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics. Blood 65: 21-31, 1985. PubMed: 3917311
|
