Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
lymphoma
Strain
BALB/c
Applications
This line was derived from the S1A lymphoma.
The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from the S1A lymphoma.
Antigen Expression
Thy-1.2
Genes Expressed
Thy-1.2
Comments
The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
K Horibata, AW Harris
Deposited As
Mus musculus
References
Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550
