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S49 (Thy-1-a)

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編號:B165648

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產品名稱	S49 (Thy-1-a)
商品貨號	B165648
Organism	Mus musculus, mouse
Tissue	lymphoma
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	BALB/c
Applications	transfection host
Antigen Expression	H-2d; TL
Genes Expressed	H-2d; TL
Comments	This variant of S49.1TB.2 (ATCC TIB-30) does not express Thy-1.2 antigen. The line was selected by 5 cycles of immunoselection using anti Thy-1 antiserum and complement. This cell line is a Class A Thy-1- mutant. The defect affects the biosysnthesis and/or assembly of the glycophospholipid membrane anchor. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	Freeze medium: Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC 4-X Storage temperature: liquid nitrogen vapor phase
Name of Depositor	R Hyman
Deposited As	Mus musculus
References	Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114 Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934 Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. PubMed: 2880714

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