| 產品名稱 |
S5 |
| 商品貨號 |
B165656 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Astrocytoma,sarcoma,sarcoma |
| Applications |
The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; gp120 glycoprotein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; gp120 glycoprotein |
| Comments |
Animals were immunized with the SK-MG-17 human astrocytoma cell line. The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons. The antigen is expressed on cultured human fibroblasts and sarcomas. In normal tissues, S5 antigen expression is limited to visceral smooth muscle. In a survey of tumors, S5 staining was limited to a subset of leiomyosarcomas and the staining was generally weaker than that with normal smooth muscle. |
| Complete Growth Medium |
Culture medium: RPMI 1640 with non-essential amino acids, 90%; fetal bovine
serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Isotype |
Secretes an IgG1 mouse monoclonal antibody. |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991
Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731
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