| 產品名稱 |
SC-71 |
| 商品貨號 |
B165671 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with type 2A myosin heavy chain in Western blots, and type IIA rat muscle fibres by immunofluorescence. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NSO myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rat type 2A myosin heavy chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rat type 2A myosin heavy chain |
| Comments |
Animals were immunized with bovine fetal skeletal muscle myosin. Spleen cells were fused with NSO myeloma cells. The antibody reacts with type 2A myosin heavy chain in Western blots, and type IIA rat muscle fibres by immunofluorescence. The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype |
IgG1 |
| Name of Depositor |
S Schiaffino |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Bottinelli R, et al. Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J. Physiol. 437: 655-672, 1991. PubMed: 1890654
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