| 產品名稱 |
SJSA-1 |
| 商品貨號 |
B165698 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
osteosarcoma; multipotential sarcoma |
| Age |
19 years adult |
| Gender |
male |
| Ethnicity |
Black |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Karyotype |
44XY; -1, -4, -7, -9, -11, -14, -15, +del(1)(q32;q42),+5p+, +der(7)t(4;HSR;7)(4qter-4q12::HSR::7p22--7qter), +9p+ and markers; amplified gli sequences were contained in the HSR in derivative No. 7 chromosomes |
| Derivation |
The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur. |
| Clinical Data |
male
19 years
Black |
| Comments |
There is amplification of the gene which encodes the p53 associated protein, MDM2.
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Two to three times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
DN Shapiro |
| Deposited As |
Homo sapiens |
| Year of Origin |
1982 |
| References |
Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537
Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305
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