| 產(chǎn)品名稱 |
SK-ES-1 |
| 商品貨號(hào) |
B165701 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Morphology |
epithelial |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma (anaplastic osteosarcoma or Ewing's sarcoma) |
| Age |
18 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 49; range = 44 to 51. This is a hyperdiploid human cell line with the modal chromosome number of 49 occurring in 34% of cells. However, cells with 50 chromosomes also occurred at high percentage (24%). At least 11 markers were consistently present in most cells. Among them were: der(3)t(3;12) (q21;q13); del(3) (q21); inv(1) (p36.1q21), and der(16)t(5;16) (q13;p13.3), all present in single copy per cell. At least three Y-autosome markers were detected. Single normal X chromosome was found in every cell. Normal Y chromosome was not found. |
| Clinical Data |
18 years Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Caucasian male Sera from normal donors were also cytotoxic. |
| Antigen Expression |
Antigen expression: Blood Type A; Rh+ |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice, forms small cell malignant tumor consistent with Ewing sarcoma |
| Comments |
Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxic. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11 D13S317: 8,9 D16S539: 11 D5S818: 12 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 14,17 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
G Trempe, LJ Old |
| Deposited As |
Homo sapiens |
| Year of Origin |
1971 |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
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