| 產品名稱 |
SK-LMS-1 |
| 商品貨號 |
B165703 |
| Organism |
Homo sapiens, human |
| Tissue |
vulva |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
leiomyosarcoma |
| Age |
43 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The cell line is aneuploid human female, with chromosome counts in the triploid to hypertriploid range. Neither of the X chromosomes are normal, but their q arms are detected in a translocation with chromosome N4. Normal chromosomes N1, N4, N13, and N17 are clearly under-represented, while chromosomes N6, N18, and N20 are consistently over-represented with respect to the copy numbers of other normal chromosomes. Eight marker chromosomes are identified: 5p+, t(1pter--->cen::9q12--->9q33::3p21--->3pter), t(1p;?), der(2)t(1;2)(q21;q24), 12p+, der(6)t(6;?)(q21;?), der(4)t(X;4)(q13;q34), del(16)(p13). The cytogenetic and isozyme phenotype descriptors are in accord with those given by J. Fogh. |
| Clinical Data |
43 years Caucasian female |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms leiomyosarcoma |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 9,10 D13S317: 12 D16S539: 8,11 D5S818: 11,13 D7S820: 8,9 THO1: 6,7 TPOX: 8,9 vWA: 18,20 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor |
G Trempe, LJ Old |
| Deposited As |
Homo sapiens |
| Year of Origin |
1971 |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031
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