亚洲.无码.制服.日韩.中I人妻无码久久精品人妻成人I制片厂91I国产真实91东北熟妇HDXXXI五月AVI四虎精品性爱I国产亚州avI日产精品久久久久I人人爱夜夜操

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > SK-MEL-2
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
SK-MEL-2
SK-MEL-2
規格:
貨期:
編號:B165706
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 SK-MEL-2
商品貨號 B165706
Organism Homo sapiens, human
Tissue skin:derived from metastasis on skin of thigh.
Product Format frozen
Morphology polygonal
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease malignant melanoma
Age 60 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype (P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Clinical Data
60 years
Caucasian
male
Antigen Expression
Antigen expression: Blood Type A; Rh+
Tumorigenic Yes
Effects
Yes, in nude mice; forms malignant melanoma
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
STR Profile Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,11
D16S539: 8,9
D5S818: 11,12,13
D7S820: 11,12
TH01: 9
TPOX: 8,9
wVA: 16,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Name of Depositor G Trempe, LJ Old
Deposited As Homo sapiens
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 五月婷婷色| 亚洲综合在线一区二区三区 | 久久亚洲综合国产精品99麻豆的功能介绍 | 91欧美视频网站 | 成人毛片在线视频 | 日韩免费一二三区 | 国产精品第一页在线 | 992tv在线成人免费观看 | 在线一二三区 | 婷婷国产在线 | 亚洲国产精品女人久久久 | 黄色一级在线视频 | 欧美三人交 | 日本黄色免费在线观看 | 一区二区欧美激情 | 亚洲女欲精品久久久久久久18 | 国产精品久久99 | 麻豆传媒在线免费看 | 色婷av | 蜜臀av在线一区二区三区 | 激情五月婷婷综合网 | 国产91全国探花系列在线播放 | 亚洲精品国产综合99久久夜夜嗨 | 91豆麻精品91久久久久久 | 色婷婷中文 | 亚洲国产午夜视频 | 在线观看视频免费大全 | 天天色天天射天天综合网 | 天天躁天天躁天天躁婷 | 国产精品高清一区二区三区 | 天天爱天天操天天干 | 欧美日本日韩aⅴ在线视频 插插插色综合 | av在线免费网站 | 欧美国产日韩在线观看 | 日韩av高潮| 深夜国产在线 | 国产一区二区在线播放视频 | 成人高清在线 | 国内精品视频久久 | 久久99亚洲精品 | 在线免费高清 | 欧美精品久久久久久久久久丰满 | av夜夜操 | 中文av在线免费观看 | 992tv成人免费看片 | 亚洲乱码精品久久久 | 91麻豆精品国产自产 | 久久,天天综合 | 亚洲va天堂va欧美ⅴa在线 | 超碰夜夜 | 不卡电影免费在线播放一区 | 欧美色就是色 | 三级黄色a| 91欧美精品 | 亚洲精品国产精品乱码不99热 | 亚洲综合色激情五月 | 久久精品一区二区三区国产主播 | 蜜臀av性久久久久av蜜臀三区 | av+在线播放在线播放 | 精品久久久久一区二区国产 | 97在线影视 | 豆豆色资源网xfplay | 国产成人精品亚洲日本在线观看 | 久久久久亚洲精品男人的天堂 | 在线视频成人 | 亚洲专区在线播放 | 国产精品精品国产 | av电影中文字幕 | 亚洲国产精品成人精品 | 日韩啪啪小视频 | 成人av影院在线观看 | 日韩av在线免费看 | 日韩1级片| 日日夜夜天天久久 | 97碰在线视频 | 婷婷视频 | 亚洲国产资源 | 亚洲成人黄色在线 | 天天综合网 天天 | 欧美精品免费在线 | 在线观看免费成人av | 91一区一区三区 | 精品欧美小视频在线观看 | 日韩三级免费 | 免费的国产精品 | 91精品国产一区 | 在线视频在线观看 | 青青河边草观看完整版高清 | 欧美亚洲成人免费 | 中文字幕av在线 | 亚洲男男gaygay无套 | 夜夜干夜夜 | 日本精品在线看 | 深爱婷婷激情 | 国产色一区 | 91在线亚洲 | 国产最新在线 | 免费在线观看国产精品 | 最新中文字幕在线资源 |