| 產(chǎn)品名稱 | SUP-T1 [VB] |
|---|---|
| 商品貨號 | B165763 |
| Organism | Homo sapiens, human |
| Cell Type | T lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | T-cell lymphoblastic lymphoma |
| Age | 8 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | 92, XXYY, -2, -2, -9, -9, the following markers are present: 2inv(2*) (p21q11), +2inv(2*), del(2*)(Q21), 2del(4)(q31q35), 2del(5)(p13p14), 2del(6)(q23q27), 2inv(14)(q11q32), t(7;9)(q34;q34), der(9)t(7;9) |
| Images |  |
| Derivation | This line was derived from malignant cells collected from the malignant pleural effusion of an 8 year old child with T-Cell Lymphoblastic Lymphoma. |
| Clinical Data | 8 years
Caucasian
male
|
| Antigen Expression | Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + Ref |
| Genes Expressed | immunoglobulin (cytoplasmic) |
| Cellular Products | immunoglobulin (cytoplasmic) |
| Comments | The cells express multiple T lineage markers. The cells are E - rosette negative and beta 2 microglobulin positive. The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) and Tac (CD25). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 x 105 viable cells/mL and maintain between 2 x105 and 2 x 106 cells/mL. Medium Renewal: 2 to 3 times per week |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | SD Smith |
| Deposited As | Homo sapiens |
| References | Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650 Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328 Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364 Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672 Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800 Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672