| 產品名稱 |
Swiss SFME (Serum Free Mouse Embryo) |
| 商品貨號 |
B165804 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Cell Type |
Astrocyte |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
clusters in suspension; the cells will attach to flasks coated with 0.02 mg/ml human fibronectin |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
embryo; 16 days |
| Strain |
Swiss |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype |
modal number = 40; range = 39 to 42 |
| Derivation |
This line was derived from minced, trypsinized Swiss mouse embryos grown in serum free medium. |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments |
Either serum or TGF-beta induce astrocyte differentiation accompanied by GFAP (glial fibrillary acidic protein) expression. Furthermore, the presence of serum causes cell growth arrest. This process is reversible upon removal of the serum. Swiss SFME cells do not grow in conventional media supplemented with fetal bovine serum or bovine calf serum. When cultured in the serum free medium (see below), Swiss SFME cells reportedly can be propagated for extended periods without undergoing"crisis" or gross chromosomal aberration. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50%;
Ham's F12 medium, 50%,
Supplements: 0.01 mg/ml bovine insulin, 0.025 mg/ml human transferrin, 0.02 mg/ml human high density lipoprotein (HDL), 100 ng/ml mouse epidermal growth factor (EGF), 10 nM sodium selenite, 1 unit/ml human platelet derived growth factor (PDGF),
and 15 mM HEPES.
NOTE: HDL is available from Sigma Chemical Co. (catalog number L2014).
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:100 is recommended Medium Renewal: Twice per week Remove medium, and collect suspended clusters by centrifugation. To disperse clusters or to remove adherent cells, add fresh 0.25% Trypsin, 0.02% EDTA at room temperature until the cells disperse (2 to 3 minutes). Add an equal volume of fresh serum free medium plus 0.1% soybean trypsin inhibitor and centrifuge at 1000 X g. Resuspend the cell pellet in growth medium, aspirate and dispense into new (fibronectin coated, if adherence is desired) flasks. Floating clusters may also be remove using a pipette and transferred to a flask containing fresh growth medium. |
| Cryopreservation |
culture medium, 80%; fetal bovine serum, 10%; DMSO, 10% |
| Name of Depositor |
Oregon State University |
| U.S. Patent Number |
|
| References |
Loo DT, et al. Extended culture of mouse embryo cells without senescence: inhibition by serum. Science 236: 200-202, 1987. PubMed: 3494308
|
