| 產(chǎn)品名稱(chēng) |
T2 (174 x CEM.T2) |
| 商品貨號(hào) |
B165813 |
| Organism |
Homo sapiens, human |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 [Cells contain Epstein-Barr virus viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This cell line is a suitable transfection host.
Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line is a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR). |
| Antigen Expression |
HLA A2; CD7 + |
| Comments |
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative.
The cells synthesize, but do not express, HLA B5.
T2 appears to have lost both copies of the CEMR.3 derived chromosome 6.
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The cells synthesize, but do not express, HLA B5.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 viable cells/mL and maintain cultures between 3 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11, 12 D13S317: 11, 12 D16S539: 10, 12 D5S818: 12, 14 D7S820: 10, 11, 15 THO1: 6, 7, 9.3 TPOX: 8, 12 vWA: 14, 17, 20 |
| Viral Testing |
Tested positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR. |
| Name of Depositor |
P Cresswell |
| Deposited As |
Homo sapiens |
| References |
Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
Salter RD, Cresswell P. Impaired assembly and transport of HLA-A and -B antigens in a mutant TxB cell hybrid. EMBO J 5(5):943-9, 1986. PubMed: 3522223
Steinle A, Schendel DJ. HLA class I alleles of LCL 721 and 174 x CEM.T2 (T2). Tissue Antigens 44(4):268-70, 1994. PubMed: 7871529
Weinzierl AO, et al. Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry. Eur J Immunol 38(6): 1503-10, 2008. PubMed: 18446792
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