| 產品名稱 |
Te39 |
| 商品貨號 |
B165845 |
| Organism |
human (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Cancer |
| Strain |
Strain:? BALB/c (myeloma) |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer. Lymphocytes were fused with NS-1 myeloma cells. |
| Clinical Data |
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor) |
| Comments |
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer. Lymphocytes were fused with NS-1 myeloma cells. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
human (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Thomson TM, et al. Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987
|
