| 產品名稱 |
TE8 |
| 商品貨號 |
B165848 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with cells forming the outer swirls of Hassall's bodies. The antibody binds specifically to cells forming the outer swirls of Hassall's bodies and also to stratum granulosum of skin epidermis and foreskin. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies) |
| Comments |
Animals were immunized with human thymic stroma. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with cells forming the outer swirls of Hassall's bodies. It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation. The antibody binds specifically to cells forming the outer swirls of Hassall's bodies and also to stratum granulosum of skin epidermis and foreskin. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum. 20%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
BF Haynes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lobach DF, et al. The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044
Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562
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