| 產(chǎn)品名稱 |
Tera-1 |
| 商品貨號(hào) |
B165850 |
| Organism |
Homo sapiens, human |
| Tissue |
testes; derived from metastatic site: lung |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
embryonal carcinoma, malignant |
| Age |
47 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
at passage 20, hypotriploid (+A, +A3, +B, +C, +D, +E, +F, -A2) with abnormalities including endoreduplications, acrocentric fragments, minutes, translocations, secondary constrictions, breaks and large metacentric markers |
| Clinical Data |
47 years adult Caucasian male |
| Tumorigenic |
No |
| Effects |
No, in immunosuppressed mice Yes, in semisolid medium |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Subculture every 6 to 8 days. |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,10 THO1: 7,8 TPOX: 8 vWA: 14 |
| Isoenzymes |
AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
J Fogh |
| Deposited As |
Homo sapiens |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797
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