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編號:B165864

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產品名稱	TIMI.4
商品貨號	B165864
Organism	Mus musculus, mouse
Tissue	thymus
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	C57BL/6
Applications	Derived from a radiation leukemia virus induced thymoma. The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml). Tested and found negative for ectromelia virus (mousepox).
Derivation	Derived from a radiation leukemia virus induced thymoma.
Antigen Expression	H-2b; Thy-1.2
Genes Expressed	H-2b; Thy-1.2
Comments	Derived from a radiation leukemia virus induced thymoma. The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml). TL antigen is not expressed. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor	R Hyman
Deposited As	Mus musculus
References	Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

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