| 產(chǎn)品名稱 | TK-1 |
|---|---|
| 商品貨號 | B165867 |
| Organism | Mus musculus, mouse |
| Cell Type | T lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | lymphoma |
| Strain | AKR/Cum |
| Applications | TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7. TK1 cells are useful for studies of alpha 4, beta 7 function and regulation. |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | TK1 is a T-cell lymphoma cell line originated by E. C. Butcher and I. L. Weissman from a spontaneous thymic lymphoma/leukemia that developed in an AKR/Cum mouse.
Ref  Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544 |
| Antigen Expression | CD8 +; alpha4, beta7 integrin + |
| Comments | They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1.
TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7. They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1. Ref The cells are CD8 positive; they display approximately 1% of L-selectin of that expressed by normal lymph node cells. |
| Complete Growth Medium | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 to 2 x 105 viable cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | EC Butcher |
| Deposited As | mouse |
| References | Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523 Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110 Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. PubMed: 6157544 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
