| 產品名稱 |
TR 310 |
| 商品貨號 |
B165877 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
The antibody reacts with a deteminant on the mouse T cell antigen receptor. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse T cell receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse T cell receptor |
| Comments |
Animals were immunized with the Tk1 murine T cell lymphoma. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a deteminant on the mouse T cell antigen receptor. It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate
and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
IL Weissman |
| Deposited As |
rat (B cell); mouse (myeloma) |
