| 產品名稱 |
TSHR-T3-365 |
| 商品貨號 |
B165890 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Derivation |
Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, intracellular domain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, intracellular domain |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the C-terminal intracellular domain (amino acids domain 604-764), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an intracellular epitope of the beta subunit anchored into the cellular membrane. The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (ATCC CRL-2684) antibody recognizes a C-terminal intracellular subunit of the beta subunit. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
H Loosfelt, E Milgrom |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295
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