| 產品名稱 | U266B1 [U266] |
|---|---|
| 商品貨號 | B165898 |
| Organism | Homo sapiens, human |
| Tissue | peripheral blood |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | myeloma; plasmacytoma |
| Age | 53 years adult |
| Gender | male |
| Applications | This cell line is a suitable transfection host. |
| Images |  |
| Clinical Data | male |
| Genes Expressed | immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6) |
| Cellular Products | immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6) |
| Comments | U266 cells have been reported to produce human IL-6. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Every 2 to 3 days |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17 |
| Isotype | IgE; lambda light chain |
| Name of Depositor | NK Nilsson |
| Deposited As | Homo sapiens |
| References | Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745 Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
