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WEHI 7.1

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編號:B165938

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產品名稱	WEHI 7.1
商品貨號	B165938
Organism	Mus musculus, mouse
Tissue	thymus
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	BALB/c
Applications	Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse. Sensitive to both cortisol and dexamethasone. Tested and found negative for ectromelia virus (mousepox).
Derivation	Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse.
Antigen Expression	H-2d; Thy-1.2
Genes Expressed	H-2d; Thy-1.2
Comments	Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse. Sensitive to both cortisol and dexamethasone. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2- 3 x 10 exp5 viable cells/ml. A saturation density of 1-2 X 10 exp6 is obtainable.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
Name of Depositor	AW Harris
Deposited As	Mus musculus
References	Pfahl M, et al. General features of steroid resistance in lymphoid cell lines. Mol. Cell. Endocrinol. 10: 193-207, 1978. PubMed: 566227 Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643 Bourgeois S, Newby RF. Diploid and haploid states of the glucocorticoid receptor gene of mouse lymphoid cell lines. Cell 11: 423-460, 1977. PubMed: 560913 Pfahl M, et al. Interaction of glucocorticoid receptors from lymphoid cell lines with their nuclear acceptor sites. Mol. Cell. Endocrinol. 10: 175-191, 1978. PubMed: 26620

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