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WIL2-NS

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編號:B165949

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產品名稱	WIL2-NS
商品貨號	B165949
Organism	Homo sapiens, human
Tissue	spleen
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Epstein-Barr virus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	hereditary spherocytosis
Age	5 years
Gender	male
Ethnicity	Caucasian
Applications	transfection host
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	The line was derived from the WIL-2 B cell line.
Clinical Data	male Caucasian 5 years
Comments	This line does not secrete immunoglobulin. The line was derived from the WIL-2 B cell line.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
STR Profile	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,12 F13A01: 6,7 F13B: 10 FESFPS: 11,12 LPL: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20
Name of Depositor	TA Coons
Deposited As	Homo sapiens
U.S. Patent Number	4,654,419
References	Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231 Alspaugh MA, Tan EM. Antibodies to cellular antigens in Sjogren's syndrome. J. Clin. Invest. 55: 1067-1073, 1975. PubMed: 804494 Vaughan JH, et al. Synthetic polypeptides and antibodies related to epstein-barr virus nuclear antigen. US Patent 4,654,419 dated Mar 31 1987 Vaughan JH, et al. Assay for Epstein-Barr virus infection with solid phase bound synthetic polypeptides. US Patent 5,116,725 dated May 26 1992 Vaughan JH, et al. Assay of anti-Epstein-Barr virus nuclear antigen antibodies with synthetic polypeptides. US Patent 5,122,448 dated Jun 16 1992 Levy JA, et al. Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines. J. Natl. Cancer Inst. 46: 647-654, 1971. PubMed: 4323138

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