| 產品名稱 |
WI-MN-1 |
| 商品貨號 |
B165951 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
The antibody reacts with antigens associated with human melanoma cells. It reacts with human melanoma cell lines and with WISH (human amnion cell line with HeLa markers) and Rc2a (human acute myelomonocytic leukemia cell line). |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3/NS1/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human melanoma associated antigens |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human melanoma associated antigens |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with the G-361 human human melanoma cell line see ATCC CRL-1424). Spleen cells were fused with P3/NS1/1-Ag4-1 myeloma cells. The antibody reacts with antigens associated with human melanoma cells. The antibody precipitates antigens of 108000 and 38000 daltons from G-361 lysates. It reacts with human melanoma cell lines and with WISH (human amnion cell line with HeLa markers) and Rc2a (human acute myelomonocytic leukemia cell line). It did not react with other line tested or with surgical specimens of non-melanoma neoplasms or with normal tissue. Most surgical specimens of melanoma were stained positively with WI-MN-1. |
| Complete Growth Medium |
Culture medium: RPMI 1640 with 2 mM L-glutamine, 1 mM pyruvate, 3.88 mg/L
thymidine, 13.61 mg/L hypoxanthine and 0.5 mg/mL gentamicin, 90%;
heat-inactivated horse serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
RPMI 1640 medium, 70%, heat-inactivated horse serum, 20%; DMSO, 10% |
| Isotype |
IgG |
| Name of Depositor |
Wadley Technologies, Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Khan A. Monoclonal antibodies to novel melanoma-associated antigens. US Patent 4,851,510 dated Jul 25 1989
|
