| 產品名稱 | Y-1 |
|---|---|
| 商品貨號 | B165978 |
| Organism | Mus musculus, mouse |
| Tissue | adrenal gland; tumor/cortex |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender | male |
| Strain | LAF1 |
| Applications | This cell line is useful for the detection of LT toxin (American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.), cholera toxin (U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995), and verotoxin. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected. |
| Images |  |
| Clinical Data | male |
| Genes Expressed | steroid hormones |
| Cellular Products | steroid hormones |
| Virus Susceptibility | Herpes simplex virus
Pseudorabies virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: At least 2 times per week (or else hormone production will decrease) |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | G Sato |
| Deposited As | Mus musculus |
| References | Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699 American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454 Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719 Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
