| 產品名稱 |
Y-17 |
| 商品貨號 |
B165981 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-E; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-E; cytotoxic |
| Comments |
Animals were immunized with Con A stimulated spleen cells from B10.A(5R) mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype |
IgG2b |
| Name of Depositor |
C Janeway |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lerner EA, et al. Monoclonal antibody against an Ir gene product. J. Exp. Med. 152: 1085-1101, 1980. PubMed: 6158546
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